Single cell suspension preparation device

The operation process of flow cytometry:

1. Extract spleen; Fix the mouse on the dissecting table and carefully cut open the entire abdominal skin from the bottom to expose the mouse peritoneum; Cut open the peritoneum of the mouse, expose the spleen, remove it, and thoroughly remove the adipose tissue on top; Cut the removed spleen into pieces and soak it in pre cooled PBS for later use

2. Prepare single cells using a single-cell suspension preparation device, set parameters, click start, and add termination solution after the program ends

3. Add ACK red blood cell lysis buffer to the centrifuged cell suspension, resuspend the cells, and lyse at room temperature for 5 minutes

4. Filter and wash through a filter screen, collect the filtered cell suspension into a centrifuge tube, centrifuge for 5 minutes, and remove the supernatant

5. Observe cells under a single-cell counting microscope, resuspend cells in staining buffer to adjust cell concentration, aspirate the cell suspension into a flow cytometer, and prepare for staining

6.Result analysis

Matters needing attention:

1. The organization should be cut into smaller pieces as much as possible for better digestion

2. After removing the spleen, try to remove the attached adipose tissue as much as possible to increase the proportion of spleen cells

3. It is recommended to use freshly prepared red blood cell lysis buffer

4. The digested cells should be washed clean, otherwise it will affect the flow cytometry effect

Characteristics of single-cell suspension preparation instrument

It is not only is it efficient, gentle, and protects cell activity, but it is also precise and controllable, adapting to different tissue types. High throughput design improves experimental efficiency. For research projects that require processing multiple samples, the high-throughput design of the single-cell suspension preparation instrument can simultaneously process multiple samples, significantly reducing experimental time. By selecting appropriate instruments, single-cell suspensions can be prepared in batches, saving experimental time. Corresponding programs for various tissues and organs of human and mouse can be selected according to the needs of the instrument, improving the viability and yield of single-cell suspension preparation, making subsequent experiments smoother.